A selection of published soft x-ray microscopy cell images and videos.
– Correlated soft x-ray and fluorescence microscopy of Schizosaccharomyces pombe showing stained vacuoles (middle image) and the segmented organelle (rightmost image) with key: nucleus (blue), nucleolus (orange), mitochondria (grey), vacuolus (white) and lipids (green). DOI: https://doi.org/10.1016/B978-0-12-374920-8.00212-5
– Combining Soft X-ray Tomography (SXT) with cryogenic Confocal Fluorescence Tomography (CFT) to combine molecular localization data with the high-resolution 3D structural images of the cell in order to identify the inactive X-chromosome.
DOI: https://doi.org/10.1016/j.bpj.2014.09.011
– Using correlative confocal live-cell imaging, cryo-fluorescence microscopy and soft x-ray tomography to link the involvement of mitochondria outer membrane proteins(MiD51) and the endoplasmic tericulum (ER) in mitochondrial fission. Key: ER extensions (green) contacting the mitochondria (red) at the MiD51 foci.
DOI: https://doi.org/10.1242/jcs.169136
– A segmented cryo-SXT image of a human fibroblast cell containing four Toxoplasma gondii parasites.
DOI: https://doi.org/10.1042/ETLS20170086
– A segmented cryo-SXT image of a breast cancer cell line (MCF-7), which had been incubated with Superparamagnetic iron oxide nanoparticles (Spion), showing fast nanoparticle transport through the endocytic pathway to the nucleus.
DOI: https://doi.org/10.1186/s12951-016-0170-4
– Soft X-ray Tomography has been used to map the reorganization of heterochromatin (type of DNA) that takes place when a stem cell matures into a nerve cell, providing a new understanding of a cell’s evolving architecture.
DOI: https://doi.org/10.1016/j.celrep.2016.10.060
– Soft X-ray Tomography was used to study hemoxoin crystal growth in the Plasmodium parasite, which is transmitted by the mosquito and causes malaria when released into the bloodstream.
DOI: https://doi.org/10.1073/pnas.1118120109