Covid Virus Tomography

SXT imaging has the ability to investigate the potential of SARS-CoV-2 virions.

The analysis of 3D architecture of infected cells is critical for understanding the mechanisms of viral diseases. Soft X-ray tomography (SXT) is a technology that can image whole intact cells in 3D under normal and pathological conditions.

Fluorescence and electron microscopy techniques, in particular, focused on beam scanning electron microscopy (FIB-SEM) have been extensively used to image the 3D structure of cells. However, although FIB-SEM achieves superior spatial resolution, the method remains low throughput. To overcome these limitations, a whole-cell imaging technique with minimal sample preparation and rapid image acquisition is required for quantitative analysis of structural changes induced in virus-infected cells.

This paper demonstrates the potential of SXT imaging by visualizing aggregates of SARS-CoV-2 virions and virus-induced intracellular alterations. This rapid whole-cell imaging approach allows us to visualize the spatiotemporal changes of cellular organelles upon viral infection in a quantitative manner.

Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells

V.Loconte,, ScienceDirect, 22 November 2021