Integrating Soft X-ray Microscopy into CLEM imaging workflows can increase cell-imaging throughput, decrease image storage requirements and provide valuable whole-cell context when analysing EM-imaged lamella.
Soft X-ray Microscopy (SXM) has traditionally only been available at a handful of synchrotrons worldwide however the development of the first commercially available lab-based soft x-ray microscope, SiriusXT’s SXT-100, has opened up new possibilities for integrating soft x-ray microscopy into cryo-correlative imaging workflows.
SiriusXT will be presenting and exhibiting its latest developments in cryo-correlative imaging workflows at the Volume EM Technology Forum, which will take place at the ESA Conference Centre, Harwell Campus, Didcot, UK, from October 22nd to 24th, 2024. SiriusXT will present on the topic of “Integrating table-top cryo soft X-ray tomography into light and electron microscopy workflows”.
The benefits of integrating SXM into a CLEM workflow (CLE’X’M) can be summarised as;
- faster cell imaging throughput
- more efficient and accurate localisation of regions of interest
- faster lamella production
- adds whole-cell volume context, facilitating image interpretation
- lower data storage requirements while retaining full 3D cell image.
SiriusXT are proud sponsors of the Volume EM Technology Forum.
