Soft x-ray tomography is an ideal imaging modality for characterizing organelle assembly within a biological cell, which informs the disease researcher about cellular mechanisms in the disease state.
In the paper below the impact of glucose stimulation on the density of insulin vesicles and mitochondria in pancreatic B-cells, under varying treatment levels and time points, is analyzed as a means of demonstrating a new automated method for segmenting cellular organelle.
The paper concludes that SXT, complemented with the segmentation of the imaged data, is a strong tool for subcellular structure recognition and drug discovery.
Auto-segmentation and time-dependent systematic analysis of mesoscale cellular structure in β-cells during insulin secretion
Angdi Li, Jitin Singla, Kate White, Valentina Loconte et.al.