Dr Kenneth Fahy, SiriusXT, presented at the EMBO workshop that took place in EMBL, covering the topic “Cryo Soft X-ray Tomography for efficient targeting and volume context in CLEM and Volume EM correlative workflows”.

Soft X-ray tomography (SXT) is a powerful standalone imaging technique with the unique capability to non-destructively image the whole 3D subcellular structures of intact hydrated biological cells or tissue samples with nanometre resolution.

SXT is also extremely beneficial when incorporated into correlative imaging workflows, by:
⭐ Identifying regions of interest (RoI) within large samples, providing precise coordinates for lamella production and subsequent TEM imaging.
⭐ Providing structural context to help analyse results by imaging a larger volume around the region of interest.
⭐ Increasing imaging through-put.
⭐ Using non-labour intensive sample preparation techniques.
⭐ Providing the ability to track and validate the delivery of nanoparticles to targeted endpoints in cells.

Check out our image gallery here to see the capabilities and applications of our SXT-100, lab-based soft X-ray microscope