Correlative HUH 7.5 cell

Seeing the Big Picture with Wide-Angle Correlative Light and X-ray Microscopy (CLXM)

SiriusXT’s SXT-100 Soft X-ray Microscope images with a wide field-of-view that offers a unique means of imaging inter- and intracellular structure of closely packed cells, whether they are in tissue, in organoids or are simply cultured in a petri dish.

The figure attached shows how the SXT-100’s integrated fluorescence microscope is used to identify two HUH7.5 cells that are close together on a TEM grid (top-left) and that are suitable candidates for Soft X-ray Tomography. An approx. 50um wide 2D soft X-ray projection (bottom-left) of these cells is used to identify an appropriate location for a full tomogram. A slice from the soft X-ray tomogram shows the high-resolution cell structure correlated with the fluorescence image, is then superimposed on the wide-angled CLEXM (right-side) image.

The extracellular matrix between cells plays a critical role in tissue architecture and function, helping to understand disease progression, particularly in cancer research where the tumour microenvironment plays a significant role in cancer progression and metastasis. Additionally, it can aid in assessing the efficacy of treatments by allowing scientists to monitor changes in cellular structures in response to therapy. Furthermore, studying the 3D intercellular space can provide insights into how organisms develop and how tissues form during embryonic development, helping to understand congenital disorders and to inform therapeutic strategies.

For more information on the SXT-100 and for more examples of soft X-ray microscopy images, please visit our product page here